Speaker: Chunzhu Zhao, Peking University

Time: 19:00 p.m., May 14, 2023, GMT+8

Venue: Tecent Meeting ID: 745-800-423 Passcode: 0514

Abstract:

Miniature multi-photon microscopy has achieved high-resolution brain imaging in freely behaving rodents. Here we report several new advances aiming for deeper, larger field-of-view and dual coloiimaging. First, we developed a 2.17-g miniature three-photon microscope with a configuration thamaximizes fluorescence collection when imaging deep in highly scattering brain tissues. It enable.noninvasive calcium imaging throughout the entire cortex and dorsal hippocampal CA1, up to 1.2 mmdepth, at a safe laser power (<50 m W). Second, we have developed a 2.5-g miniature two-photonmicroscope with a field-of-view of 1000x788 um2, comparable to that of a typical single-photonminiscope, while retaining clear resolution of neurons, dendrites and spines. By integrating adetachable 1.2-g fast z-scanning module, it allowed for a 1000 x 788 x 500 um3 volumetric imagingMore recently, through developing hollow anti-resonant fibers with broad transmission characteristicsand achromatic miniscopes, we realized dual-color two-photon or three-photon excitation atwavelengths of 780nm, 920nm and 1030 nm in near-infrared I and 1100 nm and 1300 nm innear-infrared I. These advancements will empower large-scale, dense recordings of deep brain activityin studies of neural dynamics underlying freely behaving paradigms.

Source: School of Life Sciences