Speaker: TYE Bik-Kwoon, Ph.D., Professor emeritus, Cornell University, Senior Membernstitute for Advanced Study, Hong Kong University of Science & Technology, Member of the US National Academy of Sciences

Time: 13:00-14:30 p.m., July 21, 2023, GMT+8

Venue: Deng Youcai Hall, School of Life Sciences

Abstract:

The DNA replication mechanism is highly conserved in eukaryotes yet must adapt to the diverse life styles of the myriads of species within the kingdom. I will discuss three specific DNA replication complexes, the origin recognition complex ORC [1,2], the pre-replication complex, preRc [3,4] and the MCM doublehexamer bound by its activator, the Dbf4-Cdc7 kinase [5], as examples of how theDNA replication machinery evolves to adapt to the very different life cycles adopted by yeast and human.

References:

[1] Li, N, Lam WH, Zhai Y, Cheng J, Zhao Y Gao, N and Tye, BK. Nature 559:217-222 (2018)Structure of the Origin Recognition Complex Bound to DNA Replication Origin
[2] Lee CSK, Cheung MF, LiJ, Zhao Y, Lam WH, Ho V, Rohs R, Zhai Y, Leung D, Tye BK. NatCommun 12:33 (2021) Humanizing the Yeast Origin Recognition Complex.
[3] Li, N., Zhai, Y, Zhang Y, Li, W, Yang, M., Lei, J, Tye, BK, Gao, N. Nature 524:186-91 (2015)Structure of the Eukaryotic Minichromosome Maintenance Complex at 3.8A.
[4] LiJ, Dong J, Wang W, Yu D, Fan , Hui YC, Lee CSK, Lam WH, Alary N, Yang Y, Zhang Y Zhao Q,Chen CL, Tye BK, Dang S, Zhai Y. Cel/ 186:1, 98-111.e21. (2023) The human pre-replication complexis an open complex.
[5] Cheng J, Li N, Huo Y, Dang S, Tye BK, Gao N, Zhai Y. Nat Commun 13:1396 (2022) Structuransight into the MCM Double Hexamer Activation by Dbf4-Cdc7 Kinase

Source: School of Life Sciences