Speaker: Prof. Chen Chunlai， School of Life Sciences， Tsinghua University 

Time: 14:00-15:30 p.m., Jun 27, 2024, GMT+8

Venue: B117, Research Complex #2, PKU 

Abstract: 

Single-molecule fluorescence technique utilizes highly-sensitive fluorescence microscopes to accurately measure the dynamic properties of biological macromolecules at the molecular level, to elucidate their functions and regulatory mechanisms, thereby to guide the functional modification and optimization of biomolecules. We have developed labeling, analysis and measurement tools for single-molecule fluorescence methods. With these tools, we have mainly characterized important metastable states and dynamic processes of the molecular machinery that cannot be resolved by structural biological tools to explain their molecular mechanisms, including the molecular mechanism of GPCR activation, Cas protein's target search and cleavage mechanisms and etc. These discoveries have guided us to optimize and remodel functions of Cas9, Cas12a, and GAS proteins.

Source: Biomedical Pioneering Innovation Center, PKU